Principles and Techniques of Molecular Biology

The course addresses both the fundamental principles and techniques of molecular biology. Students gain an in-depth knowledge of nucleic acid structure, molecular genetics, and the biochemistry of transcription and protein synthesis. Other topics include how mechanisms of gene regulation play a role in retroviral pathogenesis and embryonic development. Each lecture directly relates molecular biology to current laboratory techniques. Virtual laboratory sessions, using LabXchange, a free Harvard educational platform are included, These virtual laboratory sessions provide students with a broad exposure to several important techniques in molecular biology. Virtual experiments include current approaches to mutation analysis, protein interaction assays, and recombinant cDNA cloning by PCR.